Cytotoxicity Assay Analyzer Skill

Cytotoxicity Assay Analyzer

Purpose

The Cytotoxicity Assay Analyzer skill provides comprehensive in vitro toxicity assessment of nanomaterials, enabling systematic evaluation of cell viability, determination of safe exposure levels, and identification of toxicity mechanisms.

Capabilities

MTT/MTS/WST assay analysis
Live/dead staining quantification
IC50/EC50 calculation
Dose-response curve fitting
Cell morphology analysis
Apoptosis/necrosis detection

Usage Guidelines

Cytotoxicity Assessment

Assay Selection
- Choose appropriate viability assay
- Consider nanoparticle interference
- Include proper controls
Data Analysis
- Calculate percent viability
- Fit dose-response curves
- Determine IC50/EC50
Result Interpretation
- Compare to benchmark materials
- Assess mechanism of toxicity
- Report with appropriate context

Process Integration

Nanomaterial Safety Assessment Pipeline
Nanoparticle Drug Delivery System Development

Input Schema

{
  "nanomaterial": "string",
  "cell_line": "string",
  "assay_type": "mtt|mts|wst|live_dead|ldh",
  "concentrations": ["number"],
  "exposure_time": "number (hours)"
}

Output Schema

{
  "viability_data": [{
    "concentration": "number",
    "viability": "number (%)",
    "std_dev": "number"
  }],
  "dose_response": {
    "ic50": "number",
    "ic50_unit": "string",
    "hill_slope": "number",
    "r_squared": "number"
  },
  "toxicity_classification": "string",
  "mechanism_indicators": ["string"]
}

Agent Skills: Cytotoxicity Assay Analyzer

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